The ONIX™ platform uses an innovative microincubation system to maintain cells at 37°C, 5% CO₂, allow continuous medium perfusion, and prevent evaporation-- all for up to one week on the microscope stage. This approach is far more efficient than traditional stage top or enclosure control methods. The microincubation system is depicted in the figure below.

This schematic depicts the side-view of the microfluidic plate and pneumatic manifold on an inverted microscope stage. The pneumatic manifold seals to the top of the microfluidic plate wells, and enables pressure driven flow of medium to the cell chamber. It also supplies 5% CO₂ to an enclosed 1 mL chamber above the cells. The CO₂ then diffuses through the gas permeable membrane above the cells to provide the proper buffer for standard culture medium.

Temperature control is provided with an on-board circulating heater to maintain the desired setpoint (25-37°C). In cases where an oil/water immersion objective is used, an objective heater is also necessary to prevent heat loss through the lens.

Schematic not drawn to scale.