 Publication List |
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Research Lab: |
Marshall Lab, UCSF (http://biochemistry.ucsf.edu/labs/marshall/) |
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| Project Title: |
Imaging yeast cells to quantify mitochondrial morphology |
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| Authors: |
SM Rafelski, WF Marshall |
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| Testimonial: |
"I use the Onix system with the Yeast plates routinely in my imaging of live, growing yeast cells to image the mitochondria in these cells. Since I aim to quantify mitochondrial morphology, I require constant, stable imaging conditions that maintain the health of the cells, which the Onix system does very well. In addition, the system allows me to perform pharmacological or nutrient-switching timelapse experiments. I have found that the growth properties of the same cells on different days is identical in these flow chambers, which is very helpful for collecting larger data sets. Cells can continue to grow and divide until they run out of space, and survive with renewing nutrients for 1-2 days.
I have been using Cellasic plates since January, 2008 and the company has continually improved their plate design, vacuum manifold design, and added many new types of plates. They have also been exceedingly helpful in getting the system up and running and any troubleshooting that comes along." |
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Research Lab: |
Maheshri Lab, MIT (http://web.mit.edu/~narendra/www/) |
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| Project Title: |
Modification of Transcriptional Regulatory Networks in Budding Yeast |
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| Authors: |
CJ Zopf, N Maheshri |
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| Testimonial: |
“Our experience with the system and CellAsic has been excellent. We've been able to quickly and easily perform novel and technologically demanding experiments without any prior microfluidic experience. I've been able to focus on the fundamental biological questions while letting CellAsic provide me with the tools I need to answer them.”
“CellASIC is continually improving the system. We have been using their devices through many generations and have seen vast improvement and consideration of the feedback we have provided.”
“Large majority of cells loaded at the beginning of the experiment stay fixed in place for excellent time trace of single cells over long experiments (up to 16 hours in our case) Close trapping chamber positioning in Y4 reduce travel time for the stage, facilitating more frequent data acquisition. The system uses flow rates that can quickly switch media in the trapping chamber but are low enough that the reservoirs can provide for long experiments." |
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Research Lab: |
Skotheim Lab, Stanford (http://web.me.com/skotheim/Site/Welcome.html) |
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Project Title: |
Cell Cycle Commitment |
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| Authors: |
A Doncic, JM Skotheim |
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| Testimonial: |
“If you are interested in a small/medium scale system for microfluidic single-cell analysis this is a very good system which we are extremely satisfied with. Easy to work with: both to prepare the plate and to work with the software which allows us to focus on experiments and not on ‘whether the flowcell will crash after X hours.’”
“Excellent customer interaction/customer friendly representatives: before buying we were allowed to use the unit over a month to confirm that it is as good as they claimed. All-in-all it is an excellent product that we highly recommend.” |
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Research Lab: |
Marshall Lab, UCSF (http://biochemistry.ucsf.edu/labs/marshall/) |
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Project Title: |
Analyzing Ciliary Length Kinetics in Motile Chlamydomonas reinhardtiicells |
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Authors: |
WB Ludington, L Shi, Q Zhu, M Berns, W Marshall |
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Testimonial: |
"Because Chlamydomonas cells are motile, collecting length kinetics data on single cells has been difficult, if not impossible. Using the ONIX C04 microfluidic plate, we have been able to consistently collect length kinetics data on 15 cells simultaneously, with continuous control over flow speed and media composition. We are currently using the system for two projects, (1) to study the effects of flow rate and media viscosity on ciliary length, and (2) to calculate the continuous disassembly rate in the ciliary tip using length recovery from laser ablation." |
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