• Advanced microfluidic chamber design promotes cell happiness
• 4 independent chambers under a single imaging window
• Laminar flow switching between 6 inlet solutions
• Perform live-cell imaging experiments for 3+ days on the microscope stage
• 170 um thick glass bottom for high quality imaging

M04S Product Sheet
Immunostaining Cells in the M04 Plate; software protocol

The M04S is designed for high quality live cell imaging experiments with solution exchange. Six inlet channels per chamber allow multiple solution exchanges for over 3+ days. The highly laminar flow ensures rapid and uniform solution turnover on the order of seconds. (See Product Sheet for more detail.)

Continuous perfusion of culture medium to the cells ensures a suitable growth environment for long term experiments. Typical experiments run for 4-72 hours on the microscope stage, enabling single cell tracking during exposure, washout, and switching. Cells are easily loaded via pipet, and can be cultured for many days prior to experiment using a gravity driven flow in any incubator.

1. Solution Switching - Solution switching flow profiles.
2. Cell Loading - Cell loading using capillary flow.
3. Perfusion Culture - MCF-10A cells.
4. Perfusion Culture - NIH3T3 cells.
5. Perfusion Culture - PC3 cells.