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Microfluidic Open-Top Flow Chamber

Our M04O microfluidic plates enable high quality, long term live cell imaging with real-time solution switching. The open-top cell culture chamber is designed to support a wide variety of cell types, and is well suited for large cells, cell clusters/colonies, and tissues.
 
M04O Plate     M04 Hela
 
Features and Benefits:

• Advanced microfluidic chamber design promotes cell happiness
• Open-top allows direct pipetting of cell samples into chamber
• 4 independent chambers under a single imaging window
• Laminar flow switching between 6 inlet solutions
• Perform live-cell imaging experiments for 24+ hours on the microscope stage
• 170 um thick glass bottom for high quality imaging

 
Downloads:

M04O Product Sheet

 
Description:

The M04O is designed for high quality live cell imaging experiments with solution exchange. The design is similar to the M04S, but with an open top to allow direct cell dispensing. Six inlet channels per chamber allow multiple solution exchanges for over 24 hours. (See Product Sheet for more detail.)

M04O Chamber

Continuous perfusion of culture medium to the cells ensures a suitable growth environment for long term experiments. Typical experiments run for 4-48 hours on the microscope stage, enabling single cell tracking during exposure, washout, and switching. Cells are easily loaded via pipet, and can be cultured for many days prior to experiment using a gravity driven flow in any incubator.

 
Applications:

• Cell Response to Solution Change
• Induction/Inhibition
• Drug Exposures & Dose/Response

• Imaging Cell/Tissue Clusters
• Protein Translocation
• Gene Expression Dynamics

• Signal Transduction
• Cell Differentiation
• Synthetic Biology

 
Movies:

1. Solution Switching - Solution switching flow profile.
2. Perfusion Culture - Live cell imaging of MCF7 cells.

 
Image Gallery:
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M04O Hela  
Hela 7 Days, CellASIC  
 
Testimonials:
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Research Lab:
 
 
Project Title:
 
 
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Publications:
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Coming soon...

 
 

 

       
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