ONIX - Online Demonstration


	Bringing Microfluidics to Life™
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ONIX Live Cell Imaging

Perfusion Control System

MiCA Microfluidic Cell Array

PEARL Perfusion Liver System

ONIX™ Online Demonstration

The ONIX™ Microfluidic Perfusion Platform uses microfluidic technology to enable continuous live cell imaging with media flow and CO2 control. The proprietary design allows exposure changes to cells of interest via pressurized flow channels that can be alternated according to user-specified time intervals and flow rates.

1. System Components
2. Required Equipment
3. System Set-Up
4. CO2 Calibration
5. Temperature Calibration
6. Microfluidic Plate and Manifold
7. Microscope Set-Up
8. Microincubation
9. Software
10. Culture and Image Cells

1. System Components back to top

Components

The CellASIC ONIX™ platform is sold with the components displayed above.

2. Required Equipment back to top

The above laboratory equipment is required to use the ONIX™ flow system.

3. System Set-Up back to top

System Set-up

The Onix flow system is powered by any 100-240V power supply. The 100% CO2, supplied by a standard laboratory gas tank, enters the system which mixes gas to produce 5% CO2 air. The flow system connects via USB to the computer where the user can enter a desired protocol.

4. CO2 Calibration back to top

CO2calib

Optional: If the user wishes to monitor the CO2 levels during an experiment, the CO2 sensor output can be connected to the manifold with the provided tubing.

5. Temperature Calibration back to top

The temperature calibration plate plugs into the objective heater controller. Its unique design allows for precise temperature calibration of the cell culture chamber. For a more accurate measurement, turn brightfield illumination light OFF after focusing and before calibrating temperature.

6. Microfludic Plate and Manifold back to top

The manifold delivers both positive and negative pressures to each well to control fluid flow through the cell chamber. It also provides a sterile environment for the cells and serves as a conduit for CO2 delivery.

7. Microscope Set-up back to top

Place the Bioptechs objective heater onto the objective lens. Plug the heater ino the heater controller to view set temperature.The objective heater maintains a stable temperature throughout the duration of your experiment. Place plate and manifold together onto the microscope stage for live cell imaging.

8. Microincubation back to top

The manifold plate system mimics cellular environments in vivo. CO2 diffuses to the cells through a gas permeable membrane. Feedback loop and automatic stabilization features of the objective heater ensures temperature is kept at user-specified temperature.

9. Software back to top

ONIX FG software program automates your perfusion protocol for automated solution switching. The program allows the user to select which units are run, the amount of cells loaded, and the flow rate through each well.

10. Culture and Image Cells back to top

Cells

PC-3 cells stained with DAPI, phalloidin, and an anti-alpha-tubulin marker

The ONIX™is compatible with a wide variety of bacterial, yeast, and mammalian cell types. The system is adaptable to any perfusion-base experiment.