The M04 plates enable continuous perfusion culture for live cell imaging. Laminar flow provides rapid and uniform solution exchange for maximum cell health. The glass coverslide bottom surface ensures the highest resolution optical images.

Continuous perfusion of culture medium to the cells ensures a suitable growth environment for long term experiments. Typical experiments run for 4-72 hours on the microscope stage, enabling single cell tracking during exposure, washout, and switching. Cells are easily loaded via pipet, and can be cultured for many days prior to experiment using a gravity driven flow in any incubator.

1. M04S Solution Switching - Solution switching flow profiles.
2. M04S 4-Color Switching - Swithcing between 4 fluorescent solutions.
3. M04S Cell Loading - Cell loading using capillary flow.
4. M04S Perfusion Culture - MCF-10A cells.
5. M04S Perfusion Culture - NIH3T3 cells.
6. M04S Perfusion Culture - HT-1080 cells.
7. M04O Solution Switching - Solution switching flow profile.
8. M04O Perfusion Culture - Live cell imaging of MCF7 cells.
9. M04G Gradient Switching - Creating and switching a stable concentration gradient.
10. M04G Neutrophil Migration - HL-60 cells migrating in response to a spatial gradient.
11. M04G HL-60 Migration - HL-60 cells responding to a gradient.
12. M04S Transfection - HT-1080 cells in microfluidic culture tranfected with GFP-tubulin.
13. M04S Microincubation - HT-29 cells cultured for 96 hours using the ONIX Microincubator.
14. M04S Nuclear Envelope - HeLa cells treated with Nocodazole and released, GFP nuclear envelope tagged.