• Maintains stable diffusion gradients for 16+ hours
• 4 independent chambers under a single imaging window
• Laminar flow switching between 6 inlet solutions (9 gradient combinations per chamber)
• Advanced microfluidic chamber design promotes cell happiness
• Suitable for 2D and 3D gradients
• 170 um thick glass bottom for high quality imaging

M04G Product Sheet

The M04G plates utilize two microfabricated perfusion barriers to create a diffusion gradient in the culture chamber. The barriers have 4 micron pores, enabling free diffusion, but restrict convective flows. Each diffusion channel (top and bottom) is fed by 3 independent inputs, enabling sophisticated gradient switching experiments. (See Product Sheet for more detail.)

Continuous perfusion of culture medium to the cells ensures a suitable growth environment for long term experiments. Typical experiments run for 4-72 hours on the microscope stage, enabling single cell tracking during gradient exposure and switching. Cells are easily loaded via pipet, and can be cultured for many days prior to experiment using a gravity driven flow in any incubator.

1. Gradient Switching - Creating and switching a stable concentration gradient.
2. Neutrophil Migration - HL-60 cells migrating in response to a spatial gradient.
3. HL-60 Migration - HL-60 cells responding to a gradient.