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Microfluidic Flow Chamber for Chlamydomonas Cells

Our C-Series microfluidic plates keep Chlamydomonas cells fixed in place, allowing you to follow and induce cell events during high magnification imaging over many generations.
 
C04 Plate   Chlamydomonas Cells
 
Features and Benefits:

• Trap pockets hold cells in place with flow for 16+ hours
• 4 independent chambers under a single imaging window
• Laminar flow switching between 6 inlet solutions
• 170 um thick glass bottom for high quality imaging

 
Downloads:

C04 Product Sheet

 
Description:

The C04 plates utilize a microfabricated trapping pockets to hold cells in place during long term imaging. Continuous flow of media orients the flagella to the right of the trap for dynamic imaging. Each chamber contains multiple staggered trap sites to maximize loading efficiency. (See Product Sheet for more detail.)

Chlamy Plate Trap

Continuous perfusion of culture medium to the cells ensures a suitable growth environment for long term experiments. Typical experiments run for 4-16 hours on the microscope stage, enabling single cell tracking during cell division. The six inlets to each chamber allow solution switching experiments during time lapsed imaging. The highly laminar flow results in a rapid and complete solution change in under a minute.

 
Applications:

• Cell Response to Media Change
• Cell Division Tracking

• Flagella Growth
• Gene Expression

• Phase Contrast, DIC

 
Movies:

coming soon...

 
Image Gallery:
Click Here to View
Chlamy Cell  
Chlamydomonas, CellASIC  
 
Testimonials:
Click Here to View
Research Lab:
Marshall Lab, UCSF (http://biochemistry.ucsf.edu/labs/marshall/)
 
Project Title:
Analyzing Ciliary Length Kinetics in Motile Chlamydomonas reinhardtiicells
 
Authors:
WB Ludington, L Shi, Q Zhu, M Berns, W Marshall
 
Testimonial:
"Because Chlamydomonas cells are motile, collecting length kinetics data on single cells has been difficult, if not impossible. Using the ONIX C04 microfluidic plate, we have been able to consistently collect length kinetics data on 15 cells simultaneously, with continuous control over flow speed and media composition. We are currently using the system for two projects, (1) to study the effects of flow rate and media viscosity on ciliary length, and (2) to calculate the continuous disassembly rate in the ciliary tip using length recovery from laser ablation."
 
 
Publications:
Click Here to View

Coming soon...

 
 

 

       
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