• Elastic trap sites holds cells in single focal plane for 16+ hours
• 4 independent chambers under a single imaging window
• Laminar flow switching between 5 inlet solutions
• 170 um thick optical glass bottom for high quality imaging

Informational Flyer
B04A Application Note

The B04 plates utilize a microfabricated silicone ceiling with a height similar to bacteria cells to restrict their growth in a single focal plane and maintain x,y position over time. Each chamber contains 6 tapered trap heights to maximize loading efficiency of various cell sizes. The B04A plate has heights of 0.7, 0.9, 1.1, 2.0, 3.0 and 4.0 um. (See Product Sheet for more detail.)

Continuous perfusion of culture medium to the cells ensures a suitable growth environment for long term experiments. Typical experiments run for 4-16 hours on the microscope stage, enabling single cell tracking for over 10 doublings. The six inlets to each chamber allow solution switching experiments during time lapsed imaging. The highly laminar flow results in a rapid and complete solution change in under a minute.

1. E.coli Growth - Cell division of E. coli with continuous flow.
2. Solution Switching - Laminar flow exchange in the B04 chamber.
3. Caulobacter Growth - Growth of Caulobacter with continuous flow.
4. Mycobacteria Growth - Growth of Mycobacteria with continuous flow.
5. Antibiotic Burst - E. coli exposed to ampicillin.