MiCA Microfluidic Cell Array: Operation


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MiCA Operation

This page describes the basic operation of the MiCA microfluidic plate. For additional detail or questions, please call or email info@cellasic.com

0. The MiCA plate is shipped pre-primed with PBS and sealed in a sterile pack.

1. In a sterile environment, open the vacuum bag.

2. Aspirate all wells of unit(s) to be used. Each unit has 3 wells, as shown. The priming solution should be removed, leaving the liquid in the cutout at the bottom of the wells (blue outlines). The cutouts are in the shape of a square (medium inlet), circle (cell chamber), and rectangle (medium outlet).

3. Carefully remove the liquid in the cell chamber. Pipette 5ul of your cell or cell/gel solution into the cell chamber. Make sure to dispense into the hole at the bottom of the well (blue outline). A ring of PTFE tape surrounds the top of the well opening for increased hydrophobicity.

4. Add 300ul of culture medium to the Medium Inlet well. Add 30ul of liquid to the Outlet well to initiate gravity perfusion.

5. Place in a standard cell culture incubator. The MiCA is designed to perfuse the cells at a rate of 100ul/day as medium flows from the Medium Inlet to the Outlet via gravity.

6. For long term culture, refill the Medium Inlet and empty the Oulet every 2-3 days.