Key Features:

• 24 independent perfusion units
• Bio-mimetic invasion chamber design
• Use only 5 ul of gel per micro-chamber
• Continuous perfusion 3D culture
• Pump-free culture in a standard incubator
• #1.5 glass bottom for optimal imaging quality

Product Sheet
Application Note: 3D Cancer Invasion
Informational Flyer

The MiCA-Inv (M24I) microfluidic plate enables dynamic tracking of cancer invasion through 3D gel in a 96 well format. The invasion chamber is designed to mimic the in vivo microenvironment, with cells embedded in 3D ECM and a set of 8 micron pores separating the cells from continuous flow of media. Cells and gels are loaded by the user into one side of the barrier, while flow of media past the perfusion barriers allows nutrient exchange for long term culture. Solution flows from the inlet well past the cell chamber and collects in the outlet well. The flow is gravity driven and requires no external pumps, allowing the plates to be operated in a standard incubator.

(a) The M24I microfluidic plate contains 24 perfusion units on a 96 well plate. (b) Each unit has a flow inlet, a cell/gel inlet, an invasion chamber, and an outlet. (c) The invasion chamber is designed for monitoring cells crossing a gel filled invasion barrier (8 um pores) towards a continuous flow channel.

The 50 micron height chamber with thin glass bottom allows high quality live cell imaging with an inverted microscope. Cells can be observed moving through the barrier or screened at endpoints by counting the numbers on either side of the barrier. Compared with current methods for cancer invasion assay, the M24I plate offers higher quality biological information while saving time and materials.

1. Coming soon.