% M1S Gradient Switching basic protocol

setflow X     2.00
setflow Y     2.00



% Establish Gradient 1

% 0 fluorescence
open B2
% 50 fluorescence
open A1
% 100 fluorescence
open C1

%create stable gradient for one hour
wait 60



% switch to Gradient 2


close B2
close A1
close C1


% 100 fluorescence
open C2
% 50 fluorescence
open A2
% 0 fluorescence
open B1

%create stable gradient for one hour
wait 60


close C2
close A2
close B1



end