% Immunostaining Protocol

setflow X 3
setflow Y 3

% Wash with PBS
open V1
wait 15
close V1

% Fix with paraformaldehyde
open V2
wait 15
close V2

% Wash with PBS
open V1
wait 5
close V1

% Permeabilize with PBT
open V3
wait 10
close V3

% Wash with PBS
open V1
wait 5
close V1

% Block with NGS
open V4
wait 60
close V4

% Incubate with primary antibody
open V5
wait 45
close V5

% Wash with PBS
open V1
wait 15
close V1

% Incubate with secondary antibody
open V6
wait 30
close V6

% Wash with PBS
open V1
wait 10
close V1

end